Application of Mg-PLGA-rhbFGF stent on angiogenesis in hindlimb ischemia
10.3760/cma.j.issn.1673-4181.2012.01.009
- VernacularTitle:Mg-PLGA-rhbFGF复合支架在下肢缺血血运重建中的应用研究
- Author:
Hanmei BAO
;
Tianjun LIU
;
Feng LV
- Publication Type:Journal Article
- Keywords:
Mg alloy;
Recombinant human basic fibroblast growth factor,Poly(D,L-lactide-co-glycolide);
Limb ischemia;
Angiogenesis
- From:
International Journal of Biomedical Engineering
2012;35(1):33-36
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo prepare recombinant human basic fibroblast growth factor (rhbFGF) loaded magnesium-poly(D,L-lactide-co-glycolide) (Mg-PLGA) stent and to evaluate its angiogenesis effect in rat model of hindlimb ischemia.MethodsThe stent was prepared with spiral magnesium (Mg) and loaded with therapeutic agent rhbFGF and PLGA matrix.In vitro drug release study was carried out and the effect was evaluated using a standard animal model of rat hindlimb ischemia.A mechanical drill was conducted and the stent was implanted.The concentrations of Mg2+ in the muscle adjacent to the stent,rat plasma,urine and stools of the experimental animals were tested to analyze the degradation and metabolism of metal Mg.Immunohistochemical staining was performed to evaluate the angiogenesis effect of the stent.ResultsThe drug loaded in the stent could release continuously for about 4 weeks.The concentrations of Mg2+ in the rat plasma,urine and stools were within normal range.Immunohistochemical and quantitative analysis showed that the effect of Mg-PLGA-rhbFGF stent on angiogenesis of rat limb ischemia was better than that of the control group.ConclusionRhbFGF loaded MgPLGA stent could promote angiogenesis of rat limb ischemia,and it may provide theoretical basis for the critical patients suffered from lower limb ischemic disease.
