Expressions of lung cancer related genes and miRNA in peripheral blood of the residents surrounding hot springs with extremely high radon

VernacularTitle:异常高氡温泉周围居民外周血中肺癌相关基因和miRNA的表达
Author: Hongran QIN ; Mei TIAN ; Gang GAO ; Jianlei RUAN ; Jianxiang LIU
Publication Type:Journal Article
Keywords: Hot springs with high radon; p53; miR-34a/b; let-7a; k-ras
From: Chinese Journal of Radiological Medicine and Protection 2012;32(1):31-34
CountryChina
Language:Chinese
Abstract: Objective To investigate the expressions of lung cancer related genes and miRNA in peripheral blood of the residents surrounding the extremely high radon hot springs in Ruoergai County,Sichuan Province. Methods Peripheral blood samples were collected from the local residents.Expressions of lung cancer related genes (p53,k-ras) and miRNA (let-7a,miR-34a/b) were detected by real-time PCR and the protein expressions of p53 and k-ras were detected by Western blot.Results The expressions of p53 and k-ras mRNA of the residents in high radon area were 0.97 and 1.33 times of the control respectively (t =0.13,-1.12,P ＞0.05),and the p53 and k-ras protein levels were 0.70 and 1.23 times of the control respectively (t =0.72,0.46,P ＞ 0.05).The let-7a of the residents in high radon area was lower (t =1.63,P ＞ 0.05 ) while the miR-34a and miR-34b were significantly higher than those of the controls (t =- 3.20,- 3.32,P ＜ 0.05).Conclusions Based on the expressions of p53 and k-ras gene and miRNA,it can be concluded that the residents surrounding the high radon hot springs received radiation damage.