Correlation of RAD51 and radiosensitization of methotrexate
10.3760/cma.j.issn.0254-5098.2012.01.010
- VernacularTitle:氨甲蝶呤辐射增敏作用与RAD51的相关性研究
- Author:
Liqing DU
;
Jianqiang BAI
;
Qiang LIU
;
Yan WANG
;
Peng ZHAO
;
Fenghua CHEN
;
Hong WANG
;
Feiyue FAN
- Publication Type:Journal Article
- Keywords:
RAD51;
Methotrexate;
Radiosensitization
- From:
Chinese Journal of Radiological Medicine and Protection
2012;32(1):44-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the correlation between homologous recombination repair protein RAD51 and methotrexate-enhanced radiosensitivity.Methods Western blot and RT-PCR assays were used to detect RAD51 expression in HOS osteosarcoma cells exposed to γ-ray irradiation alone and in combination with methotrexate.Colony formation assay was used to test the survival fraction of HOS cells exposed to γ-rays and methotrexate.Results Methotrexate inhibited both protein and RNA expressions of RAD51,and the combination of radiation and methotrexate enhanced the inhibition of RAD51 expression.Moreover,transfection of cells with RAD51 gene decreased cellular sensitivity to methotrexate and γ-rays.The sensitizer enhancerment ratios after irradiation in combination with methotrexate were 1.51 and 0.99,respectively.Methotrenate was a preferred radiosensitizer to HOS cell.Conclusions RAD51 might be involved in the methotrexate-enhanced radiosensitivity.
