Effect of electromagnetic radiation at different bands on the expressions of SOX9 and WT1 in rat Sertoli cells
10.3760/cma.j.issn.0254-5098.2012.01.011
- VernacularTitle:不同波段电磁辐射致大鼠Sertoli细胞SOX9和WT1表达的影响
- Author:
Hui WU
;
Dewen WANG
;
Shuiming WANG
;
Hongyan ZUO
;
Yang LI
;
Yabing GAO
;
Xinping XU
;
Hongmei ZHOU
;
Zhentao SU
;
Hua YAO
;
Ruiyun PENG
- Publication Type:Journal Article
- Keywords:
Electromagnetic radiation;
Sertoli cells;
SOX9;
WT1
- From:
Chinese Journal of Radiological Medicine and Protection
2012;32(1):47-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of SOX9 and WT1 expressions in rat Sertoli cells irradiated by EMP ( electromagnetic pulse),S-HPM ( S-band high power microwave) and X-HPM ( Xband high power microwave).Methods Primary Sertoli cells were isolated from 3-week-old Wistar rats and its purity was immunocytochemistrically indentified with WT1.After exposure to 6 × 104 V/m EMP,100 mW/cm2 S-HPM and X-HPM for 4 min respectively,SOX9 and WT1 expressions in Sertoli cells were determined with real-time PCR and Western blot,respectively.Results SOX9 mRNA expression was decreased at 6 and 12 h post-irradiation of three different bands of electromagnetic microwave ( F =15.20and 4.84,P ＜ 0.05 ).SOX9 protein expression was also decreased at 6 and 24 h after irradiation ( F =8.46 and 7.47,P＜0.05).WT1 mRNA expression was decreased at6 and 12 h (F=13.46 and 5.08,P ＜ 0.05 ),but its protein expression was decreased only at 24 h post-irradiation ( F =10.26,P ＜ 0.05 ).Conclusions Three bands of electromagnetic radiation reduce the expressions of SOX9 and WT1 in rat Sertoli cells,which may provide molecular foundation for genital system hazards induced by microwave radiation.
