Effect of high power pulse microwave on morphological changes of pancreas and nitric oxide and endothelin in blood serum in rats
10.3760/cma.j.issn.0254-5098.2012.01.012
- VernacularTitle:高功率脉冲微波辐照对大鼠胰腺及血清一氧化氮和内皮素的影响
- Author:
Binghua ZHANG
;
Jinxiu FEI
;
Yongbo GUO
;
Hongxia WANG
;
Xiaofeng SONG
;
Qinsheng WEN
;
Yuxin HUANG
- Publication Type:Journal Article
- Keywords:
High power pulse microwave;
Nitric oxide;
Endothelin;
Pancreas
- From:
Chinese Journal of Radiological Medicine and Protection
2012;32(1):52-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the morphological changes of rats' pancreas and nitric oxide (NO) and endothelin(ET) in the blood serum in rats after exposure to different pulses of high power pulse microwave (HPPMW).Methods SD-rats were irradiated with 104,105 and 4 × 105 pulses of HPPMW,respectively.After gloss observation,the histopathological changes of pancreas were observed through biological microscope and electroscope.The changes of amylase,nitric oxide and endothelin in blood serum were detected by biochemical and radio-immunological methods. Results Compared with the blank control,no apparent abnormality could be observed in the pancreas of all groups.The dilatation of capillary could be observed in each experimental group by microscope.The ultrastructure changes of pancreas were most serious in 4 × 105 pulse group,especially at 24 and 48 h after irradiation.Compared with the control group,the levels of serum amylase were decreased (F =12.58,11.73,P ＜ 0.05),while ET were increased (F =4.50,4.49,P ＜0.05) at 24 and 48 h after irradiation.The levels of NO in serum were increased ( F =17.51,41.72,19.98,32.64,P ＜ 0.05 ) at each time-point.The level of NO went up with the increase of pulses.Conclusions HPPMW has damage effects on the pancreas in rats.The pulses with the pancreas can lead to severity of the damage. The mechanism of HPPMW may be involved in the enhancement of ET and NO in serum.
