Correlation between acquired drug resistance-related genes and mobile genetic elements in pandrugresistant Acinetobacter baumannii
10.3760/cma.j.issn.1674-2397.2012.01.003
- VernacularTitle:泛耐药鲍曼不动杆菌获得性耐药基因和可移动遗传元件检测与指标聚类分析
- Author:
Weihua WANG
;
Jie CHEN
;
Xiongying MAO
;
Wanfei Lü
;
Li WANG
;
Hui CHEN
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Pandrug resistance;
Acquired resistance-related genes;
Mobile genetic elements;
Polymerase chain reaction;
Cluster analysis
- From:
Chinese Journal of Clinical Infectious Diseases
2012;05(1):9-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between acquired drug resistance-related genes and mobile genetic elements from pandrug-resistant Acinetobacter baumannii. Methods Fifty-three horizontal transfer drug resistance-related genes ( β-lactamases,aminoglycoside and quinolones resistance related) and 12 mobile genetic elements (including zygosity plasmid,transposon,insertion sequence and integron) were detected by polymerase chain reaction (PCR) in 20 clinical isolates of pandrug-resistant Acinetobacter baumannii.Index cluster analysis was performed to explore the correlation.Results In 20 strains of pandrug-resistant Acinetobacter baumannii,there were 3 types of β-lactamases related genes (TEM-1,ADC-30,OXA-23 ),4 types of aminoglycoside modifying enzyme genes [ aac (3)-Ⅰ,aac(6′)-Ⅰ b,ant( 3″)-Ⅰ and aph( 3′)-Ⅰ ],and 5 kinds of mobile genetic elements ( int Ⅰ 1,tnpU,tnp513,IS26 and ISAba1 ). Index cluster analysis showed high correlations between resistance genes [TEM-1,ADC-30,aac( 6′)-Ⅰ b,ant( 3″)-Ⅰ,abeB,qacE Δ1] and mobile genetic elements ( int Ⅰ 1,tnpU,tnp513,IS26,ISAba1 ).Conclusion Clinical isolated pandrug-resistant Acinetobacter baumannii carries several acquired drug resistance-related genes and mobile genetic elements,and there may be a close association between them.
