Effect of anti-oncogene PTEN on cell proliferation in breast cancer cell BT549
10.3760/cma.j.issn.1008-1372.2011.z2.006
- VernacularTitle:稳定转染抑癌基因PTEN对乳腺癌细胞BT549生长增殖的影响
- Author:
Zuo YING
- Publication Type:Journal Article
- Keywords:
Anti-oncogene;
PTEN;
Breast cancer;
Cell proliferation
- From:
Journal of Chinese Physician
2011;02(z2):16-19
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of PTEN on cell proliferation in human breast cancer cell BT549.MethodsThe plasmid pcDNA3-PTEN was used to transfect the PTEN - null breast cancer cell BT549 by lipo - transfection.After G418 selection,BT549 cells which stably expressed PTEN were obtained and amplified.Western blot were used to determine the target protein expression,the cell viability was tested by MTT assay.Results(1)Compared with the control,PTEN-BT549 cells demonstrated high expression of PTEN protein ;(2)The proliferation speed of PTEN-BT549 was obviously slower than BT549 and pcDNA3-BT549 cells( P ＜ 0.05 ).ConclusionAnti-oncogene PTEN suppresses the growth of breast cancer cell BT549.
