The effects and mechanisms of BTBD10 on the proliferation of islet beta cell
10.3760/cma.j.issn.0578-1426.2012.02.016
- VernacularTitle:BTBD1O对胰岛β细胞增殖的影响和作用机制研究
- Author:
Yu LIU
;
Zhaoyan GU
;
Xinyu MIAO
;
Yahping GONG
;
Yujun XIAO
;
Jian LI
;
Hui TIAN
;
Chunlin LI
- Publication Type:Journal Article
- Keywords:
Insulin-secreting cells;
Cell proliferation;
BTBD10
- From:
Chinese Journal of Internal Medicine
2012;51(2):136-139
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the role of BTBD10 overexpression in the proliferation of insulinoma cell line INS-1and its mechanism. MethodsThe recombined expression plasmid of pcDNA4.0-BTBD10 was constructed by gene cloning technique and was transfected into INS-1 cell by lipofectamine 2000. The stable overexpression BTBD10 of INS-1cell was selected at 48th hour after transfection.INS-1 cell proliferation activity was measured by MTT method.The expression of BTBD10,protein kinase B(Akt),phospho-Akt(p-Akt),mammal target of rapamycin (mTOR) and phospho-mTOR (p-mTOR) were determined by Western blot.ResultsThe stable overexpression BTBD10 of INS-1 cell wassuccessfullyconstructed.OverproductionofBTBD10promotedbetacellproliferation.The phosphorylation of Akt and mTOR was increased and the ratio of p-Akt/Akt and p-mTOR/mTOR was enhanced in the INS-1 overexpressed by BTBD10.But the expression of total Akt and mTOR presented no obvious changes. Conclusion The overexpression BTBD10 of INS-1 cell could activate of Akt/mTOR signalling pathway via stimulating phospho-mTOR and Akt,and enhance overall cell protein translation,so as to promote proliferation of INS-1 cell.
