Polymorphisms in the interleukin-1 receptor-associated kinase 4 gene are associated withsusceptibility to severe sepsis
10.3760/cma.j.issn.1671-0282.2012.02.005
- VernacularTitle:白介素-1受体相关蛋白酶-4基因多态性与脓毒症的易感性
- Author:
Chenling YAO
;
Chenglong LIU
;
Zhenju SONG
;
Jun YIN
;
Chaoyang TONG
;
Peizhi HUANG
- Publication Type:Journal Article
- Keywords:
Severe sepsis;
IRAK4;
Single nucleotide polymorphism;
Susceptibility;
Prognosis;
Chinese Han population;
Allele;
Genotype
- From:
Chinese Journal of Emergency Medicine
2012;21(2):128-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible association of IRAK4 polymorphisms with susceptibility to and prognosis of severe sepsis.Methods A total of 192 patients hospitalized in emergency department of Zhongshan Hospital from February 2006 to December 2009,and another 192 healthy volunteers were enrolled in this case-control study.Patients were excluded if they had metastatic tumors,autoimmune diseases,AIDS or received immunosuppressive drugs.This study was approved by the ethical committee of Zhongshan Hospital,Fudan University.Sepsis patients were divided into survival group(n =124)and non-survival group(n =68)according to the 30-day mortality.Primer 3 software was used to design the PCR and sequencing primers.Genomic DNA was extracted from peripheral blood mononuclear cells.Seven tagSNPs were selected based on the data of Chinese Han in Beijing from the Hapmap projectand genotyped by direct sequencing.We used x2 analysis to evaluate the significance of differences in genotype and allele frequencies between different groups.Results The distributions of all tagSNPs were consistent with Hardy-Weinberg equilibrium.The allele and genotype frequencies of rs4251545(G/A)were significantly different between severe sepsis and healthy control groups(P =0.015,P =0.035,respectively).Carriers of the rs4251545A had a higher risk for severe sepsis compared with carriers of the rs4251545G(OR =1.69,95％ CI:1.10-2.58).The allele and genotype frequencies of all SNPs were not significantly different between survivor group and non-survivor group.Conclusions These findings indicated that the variants in IRAK4 are significantly associated with severe sepsis susceptibility in the Chinese Han population.
