Cerebral ischemic postconditioning attenuate cerebral ischemic reperfusion injury
10.3760/cma.j.issn.1671-0282.2012.02.015
- VernacularTitle:缺血后处理减轻脑皮质缺血-再灌注损伤
- Author:
Liying ZHAN
;
Bo ZHAO
;
Zhongyuan XIA
- Publication Type:Journal Article
- Keywords:
Ischemic postconditioning;
Reperfusion injury;
Cortex;
Neuron;
Apoptosis;
GlycogenSynthase Kinase-3β;
Phosphorylation;
Brain protection
- From:
Chinese Journal of Emergency Medicine
2012;21(2):171-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess effects of cerebral ischemic postconditioning(IPost)on neuronal apoptosis and phosphated glycogen synthase kinase-3β(GSK-3β)after cerebral ischemic/reperfusion.Methods The experiment was conducted at the center for animal experiment of Renmin Hospital,Wuhan University.Thirty male Wistar rats were randomly divided into three groups:sham operation(S),ischemic/reperfusion(I/R)and ischemic postconditioning(IPost).Each group contained ten rats.Global brain ischemia was produced with four-VO method.Animals were killed after two days.Apoptosis in neurons in the cortex region were detected by TUNEL assay; infarct areas were detected by TTC ; activity of p-GSK-3β was detected by spectrum assay; Statistical software SPSS13.0 was applicated to perform one-way ANOVA Student-Newman-Keul test; correlation was detected by linear regression.Results Compared with group S,TUNEL-positive cells and infarct areas increased(P ＜0.01),the activity of p-GSK-3β decreased in I/R and IPost groups(P ＜ 0.01).Compared with group I/R,TUNEL-positive cells and infarct areas significantly decreased(P ＜ 0.01),the activity of p-GSK-3β increased in group IPost(P ＜ 0.01).The activity of p-GSK-3β and TUNEL-positive cells had highly correlation,so as infarct areas(P ＜ 0.01).Conclusions IPost can lessen the ischemic/reperfusion injury of Cortex,through increas the activity of p-GSK-3[β and decreasing neuronal apoptosis.
