Effect of decoy receptor-3 and glutamic acid decarboxylase-65 recombinant adenovirus on type 1 diabetes mice

VernacularTitle:诱骗受体3和谷氨酸脱羧酶65重组腺病毒对糖尿病小鼠的作用研究
Author: Min ZHANG ; Shufa LI ; Bing CHEN ; Jianyong ZHAO ; Chao JIANC
Publication Type:Journal Article
Keywords: Decoy receptor-3; Glutamic acid decarboxylase-65; Diabetes mellitus,experimental; Gene therapy
From: Chinese Journal of Endocrinology and Metabolism 2012;28(2):150-152
CountryChina
Language:Chinese
Abstract: The decoy receptor-3 ( DcR3 ) and glutamic acid decarboxylase-65 ( GAD65 ) recombinant adenovirus was construced and transduced into denlritic cells (DC). After the transduced DC were utilized to immunize NOD mice,the CD+8 T cells and blood glucose were analyzed. The results showed that recombinant adenovirus inhibited the proliferation and cytokine release of GAD65 specific T cells,and delayed the incidence of diabetes.Both interferon-γ[ (50.5±7.2)vs(95.4±6.9) and(91.2±6.5) pg/ml] and interleukin-2 [ (46.3±5.1 )vs ( 86.1 ±5.2 ) and ( 80.3 ± 7.3 ) pg/ml ] were decreased compared to those in negative and blank controls ( all P＜0.05 ).The results suggest that DcR3 and GAD65 recombinant adenovirus might provide a promising way for gene therapy of type 1 diabetes.