Detection of human papillomavirus DNA in extragenital Bowen's disease by using a nested PCR
10.3760/cma.j.issn.0412-4030.2012.02.010
- VernacularTitle:巢式PCR方法检测非生殖器部位Bowen病HPV DNA
- Author:
Yinghui KONG
;
Yeping WEI
;
Shuyun ZHENG
- Publication Type:Journal Article
- Keywords:
Bowen's disease;
Human papillomavirus;
Polymerase chain reaction;
Sensitivity and specificity
- From:
Chinese Journal of Dermatology
2012;45(2):99-101
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo develop a new nested PCR to detect human papillomavirus (HPV) DNA in lesions of extragenital Bowen's disease.Methods DNA was extracted from the lesions of 41 patients with extragenital Bowen's disease and suhjected to the amplification of HPV by a nested PCR.Five primers,including CN1FR,CN2FR,CN3FR,CN4FR and CN5FR,were designed and used in the second round PCR.ResultsBased on the ClustalX analysis,69 HPV subtypes,including mucosal types,cutaneous types and epidermodysplasia verruciformis-associated types,could be amplified by using the 5 designed primers.The detection limit varied from 10-3 to 10-2 copies of HPV DNA for this PCR.Of the 41 lesional specimens,5 were positive for HPV DNA,including 3 cases of high-risk HPV types(2 cases of HPV 16,1 case of HPV 33) and 2 cases of cutaneous HPV types(1 case of HPV 27 and 1 case of HPV 76).ConclusionsThe improved nested PCR is highly sensitive and specific for the detection of HPV DNA in lesions of extragenital Bowen's disease.The development of extragenital Bowen's disease may be associated with the infection with high-risk mucosal HPV types in some patients.
