Variation of peripheral blood CD34 + cells during hematopoietic stem cell mobilization and its influence on collection
10.3760/cma.j.issn.0254-1785.2012.02.006
- VernacularTitle:造血干细胞动员中外周血CD34+细胞的变化趋势及其对采集的影响
- Author:
Yehui TAN
;
Xiaoliang LIU
;
Chang WANG
;
Jingnan SUN
;
Xiumin SHI
;
Wei HAN
;
Zhonghua DU
;
Long SU
;
Sujun GAO
;
Wei LI
- Publication Type:Journal Article
- Keywords:
Hematopoietic stem cell mobilization;
CD34 + cells;
Stem cell collection
- From:
Chinese Journal of Organ Transplantation
2012;33(2):90-93
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the variation of peripheral blood CD34+ cells during the hematopoietic stem cell mobilization,and its influence on the collecting timing and results.Methods Twenty-seven cases of peripheral blood hematopoietic stem cell mobilization and collection from April 2010 to December 2011 were analyzed,including 13 autologous cases mobilized with chemotherapy combined with granulocyte colony-stimulating factor (G-CSF,10 μg· kg-1 · d-1) and 14 cases of healthy donors mobilized with only G-SCF (7.5 μg · kg- 1 · d- 1 ).The number of peripheral blood CD34+ cells was counted,and its correlation with the yield of mononuclear cells (MNCs) and CD34+cells was analyzed.ResultsMNCs (5.84 ± 1.48) × 108/kg and CD34+ cells (3.93 ± 2.16) × 106/kg were obtained in healthy donors,and (6.58 ± 3.72) × 108/kg MNCs and (3.98 ± 3.06) × 106/kg CD34+ cells were obtained in autologous cases,respectively.There was only 1 failure in autologous cases.The peak of peripheral blood CD34+ cells in autologous cases appeared at day 4 after the treatment of G-CSF,and in healthy donors the number of peripheral blood CD34+ cells at day 5 was still in ascendant phase.The CD34+ cells/kg in the collection products were positively correlated with the percentage and absolute value of peripheral blood CD34+ cells.The cases ratio of CD34+ cells≥2× 106/kg in the products of single collection was up to 76.2％ (16/21) in the cases with peripheral blood CD34+ cells absolute value greater than 20/μl.ConclusionThe number of peripheral blood CD34+ cells was an important monitoring indicator in hematopoietic stem cell mobilization and collection,CD34+ cell absolute value ≥20/μl could be used as collection threshold.
