Poly (ADP-ribose) polymerase inhibitor attenuates expression of inflammatory mediators and injury of lung in a rat model of severe acute pancreatitis
10.3760/cma.j.issn.1671-0282.2012.01.009
- VernacularTitle:聚ADP核糖聚合酶抑制剂对重症急性胰腺炎大鼠肺炎症介质表达和肺损伤的影响
- Author:
Jia YU
;
Chen CHEN
;
Weixing WANG
;
Wenhong DENG
;
Xiaoyan CHEN
;
Ying CHEN
- Publication Type:Journal Article
- Keywords:
Severe acute pancreatitis;
Lung injury;
Poly (ADP-ribose) polymerase inhibitor;
3-Aminobenzamide;
Inflammatory mediators
- From:
Chinese Journal of Emergency Medicine
2012;21(1):38-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of poly-ADP-ribose polymerase inhibitor 3-aminobenzamide (3-AB) on lung injury in rats with severe acute pancreatitis (SAP) and to explore the mechanisms.Methods Thirty-six Wistar rats were randomly (random number) divided into three groups (n =12 for each group),namely sham operation (SO) group,SAP group and 3-AB-treated group.The model of SAP-associated lung injury was established by retrograde injection of 5％ sodium taurocholate (STC) into the biliopancreatic duct.In the treated group,3-AB in dose of 10 mg/kg was administered twice by intravenous injection 30 min before and 30 min after STC infusion.The survival rats were sacrificed 12hours after SAP modeling,and the serum amylase,lung wet/dry ratio and myeloperoxidase (MPO) activity were determined,and pathological scores of pancreas and lung tissue were evaluated under light microscope.Expressions of interleukin (IL) -1 β and IL-6 mRNA,tumor necrosis factor α (TNF-α) and inter-cellular adhesion molecule-1 (ICAM-1) protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot,respectively. Results The serum amylase level,lung wet/dry ratio and MPO activity,IL-1β and IL-6 mRNA expressions,TNF-α and ICAM-1 protein levels were dramatically increased in SAP group ( P ＜ 0.05 ).Treatment with 3-AB significantly reduced these biomarkers in 3-AB group than in SAP group (P ＜ 0.05 ).Conclusions Poly-ADP-ribose polymerase inhibitor 3-AB exerts the protective and therapeutic effects on lung injury associated with severe acute pancreatitis through inhibiting intrapulmonary MPO activity and down-regulating the expressions of IL-1 β and IL-6 mRNA as well as the levels of TNF-α,and ICAM-1.
