Glucosamine sulfate inhibits nitric oxide synthesis induced by IL-1 beta in human osteoarthritic chondrocytes
10.3760/cma.j.issn.0254-9026.2012.02.015
- VernacularTitle:硫酸氨基葡萄糖抑制白介素1β诱导的人骨关节炎软骨细胞合成一氧化氮研究
- Author:
Xiaobin WANG
;
Gongyi HUANG
;
Qingyun XUE
- Publication Type:Journal Article
- Keywords:
Amino sugars;
Osteoarthritis;
Chondrocytes;
Nitric oxide
- From:
Chinese Journal of Geriatrics
2012;31(2):140-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of glucosamine sulfate on nitric oxide(NO)production induced by interleukin(IL)-1β in human osteoarthritis chondrocytes(HOC),and explore the possible mechanism.Methods Chondrocytes were harvested from 10 osteoarthritis patients undergoing total knee replacement(TKR)operation.Human recombinant IL-1β(5 μg/L)and glucosamine sulfate GS in different concentrations(0.2 mmol/L,2.0 mmol/L,20.0 mmol/L)were administrated into cell culture medium for 24 h.The content of NO was detected by enzyme-linked immunosorbent assay(ELISA).The mRNA and protein expression of inductive nitric oxide synthetase(iNOS)were measured by RT-PCR and Western blot,respectively.Results Stimulation of HOC with IL-1β enhanced production of NO and expressions of iNOS mRNA and protein(t=-14.81,-45.38,all P＜0.01).Pretreatment with 2.0 and 20.0 mmol/L GS showed a dose-dependent inhibition of IL-1β induced NO production(F=12.43,P＜0.05)and the expression levels ofiNOSmRNA(F=142.28,P＜0.05)and protein(F=78.08,P＜0.01).20.0 mmol/L GS alone did not influence NO production(t =-0.17,P＞ 0.05).Conclusions GS may inhibit the synthesis of NO induced by IL-1β in HOC through down-regulate mRNA and protein expressions of iNOS.
