Clinical significance of human cytomegalovirus pp67-mRNA in renal transplant recipients
10.3760/cma.j.issn.1008-1372.2012.01.004
- VernacularTitle:pp67-mRNA在肾移植术后人巨细胞病毒感染中的诊断价值
- Author:
Chuan ZHANG
;
Yong SHAO
;
Zhizhong GAO
;
Minggang ZHANG
;
Wentao WANG
;
Yunli LIU
- Publication Type:Journal Article
- Keywords:
Viral matrix proteins/BL;
Cytomegalovirus infections/BL/DI/DT;
Kidney transplantation/AE;
Antiviral agents/TU
- From:
Journal of Chinese Physician
2012;14(1):9-12
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the relation of the human cytomegalovirus pp67-mRNA with the active infection in renal transplant recipients and the role in the direction for antiviral therapy.Methods Total 128 samples were collected from peripheral blood treated with EDTA of 32 recipients at the 3rd week or the 7th week after transplantation.IFA was used to detect CMV pp65 antigen,and NASBA was used to detect pp67 mRNA assay.Compared with CMV-Ag,the recipients were followed up to observe the change of pp67 in the cases of the active infection and the CMV disease.ResultsCompared with the proportion between two groups,44 samples out of 128 samples showed CMV-Ag pp65 positive,while pp67-mRNA were positive in 21 samples.8 patients had been found positive CMV-Ag pp65 and pp67-mRNA; 12 recipients were clinically diagnosed with HCMV disease.In the early stage of the HCMV infection,the sensitivity and specificity of the CMV-Ag pp65 ( 91.7％,50.0％ ) was higher than that of pp67-mRNA ( 66.7％,95.0％ ).During the periods of symptoms appeared and the late stage of clinical treatment,the specificity of pp67-mRNA was higher than that of CMV-Ag pp65,while the sensitivity sit on the contrary.And in the antiviral therapy course,the indicator of antiviral therapy pp67 turned negative more quickly than the CMVAg,the therapy course could be reduced.ConclusionsBoth of CMV-Ag pp65 and of pp67-mRNA have clinical significance.Testing for CMV-Ag pp65 is suitable for detecting HCMV active infection at the early stage,which was important for in time anti-virus treatment in the clinical settings.The test for pp67-mRNA is a quick method to accquire the accurate results; therefore,it can be used as the reference criteria for the clinical anti-virus treatment.
