An in vitro study of rhBMP-2/ vancomycin/ calcium sulfate drug delivery system
10.3760/cma.j.issn.1671-7600.2012.01.014
- VernacularTitle:重组人骨形态发生蛋白-2/万古霉素/硫酸钙药物缓释系统体外释放的实验研究
- Author:
Longbao ZHENG
;
Xinqiang WANG
;
Hang LI
;
Zhijun PAN
- Publication Type:Journal Article
- Keywords:
Bone morphogenetic proteins;
Vancomycin;
Calcium sulfate
- From:
Chinese Journal of Orthopaedic Trauma
2012;14(1):53-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the efficacy and safety of in vitro local drug delivery capsules fabricated with the biodegradable material Osteoset(R)plus vancomycin and recombinant human bone morphogenetic protein-2 (rhBMP-2). Methods In vitro local drug delivery capsules were fabricated with the biodegradable material Osteoset(R) plus vancomycin and rhBMP-2.High-Performance Liquid Chromatography (HPLC) and bacterial inhibition tests were performed to detect the concentration and activity of vancomycin in the capsules.Enzyme-linked immunosorbent assay(ELISA) and alkaline phosphatase(ALP) tests were conducted to detect the concentration and activity of rhBMP-2 in the capsules. Results The capsules could release vancomycin above 55.8 μg/mL for up to 144 hours,with an activity of above 70％.They could release active thBMP-2 for up to 30 days,had no inhibition effects on the proliferation of mesenchymal stem cells(MSCs) and had high biological safety. Conclusion Since the capsules of Osteoset(R) plus vancomyein and rhBMP-2 can release high concentrations of active vancomycin and active rhBMP-2 with no inhibition of the proliferation of MSCs,they have good prospects for clinical use.
