Insl3 receptor (LGR8)expressed on the acrosome of spermatocytes and sperm

VernacularTitle:胰岛素样因子3受体(LGR8)在生精细胞与精子顶体中表达
Author: Yi WANG ; Shaohua ZHANG ; Bingyin SHI ; Baker LINDA
Publication Type:Journal Article
Keywords: Insulin like factor-3; Leucine-rich repeat-containing G protein-coupled receptor 8; Cryptorchidism; Sperm; Acrosome
From: Chinese Journal of Endocrinology and Metabolism 2012;28(2):156-159
CountryChina
Language:Chinese
Abstract: Immunoprecipitation and Western-blot were performed to detect the expression of LGR8 in the testes in wild type (Wt) and Insl3-knockout ( KO ) mice.Testis were harvested from WT and KO mice on embryonic day 18 (E18) and on postnatal(P) 1,3,7,14,17,20,23,51,and 90 days.LGR8 staining in Leydig cells was stronger in all Wt mice than that in KO mice( all P＜0.05 ).LGR8 expression on spermatocytes varies with age.Strong stain on P1 Wt and postpubertal mice was concentrated in the developing and mature acrosome of spermatids