Role of circulating microRNA-22 in pathogenesis of idiopathic pulmonary arterial hypertension
10.3760/cma.j.issn.1671-0282.2012.01.013
- VernacularTitle:microRNA-22在特发性肺动脉高压发病机制中的作用
- Author:
Chao ZHENG
;
Qun LIU
;
Zhengrong HUANG
;
Rong TANG
;
Wuyang ZHANG
;
Ziguan ZHANG
;
Delong LI
;
Zhipeng ZENG
- Publication Type:Journal Article
- Keywords:
Idiopathic pulmonary hypertension (IPAH);
MicroRNA-22;
Biomarker;
Myc binding protein (MYCBP)
- From:
Chinese Journal of Emergency Medicine
2012;21(1):57-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the value of plasma miR-22 in diagnosis of idiopathic pulmonary arterial hypertension ( IPAH ),and its role of regulation mechanisms in the pathogenesis of the disease.Methods Circulating miR-22 levels of IPAH patients and healthy controls were evaluated by RTPCR.The silico analysis of targets for miR-22 was taken, and followed by eGFP reporter assay for verification of predicted target gene Myc binding protein (MYCBP). Results Compared with healthy controls,the expression of plasma miR-22 in IPAH patients was significantly decreased (P < 0.01 ).The area under curve (AUC) of ROC curve was 0.744.MYCBP was a real target of miR-22 confirmed by silico analysis and eGFP reporter assay. Conclusions The expression of plasma miR-22 was significantly decreased in IPAH patients,and it could serve as a potential biomarker for diagnosis.The miR-22 might be involved in the pathogenesis of the disease through promoting its target gene MYCBP to activate the c-Myc pathway.