Role of circulating microRNA-22 in pathogenesis of idiopathic pulmonary arterial hypertension

VernacularTitle:microRNA-22在特发性肺动脉高压发病机制中的作用
Author: Chao ZHENG ; Qun LIU ; Zhengrong HUANG ; Rong TANG ; Wuyang ZHANG ; Ziguan ZHANG ; Delong LI ; Zhipeng ZENG
Publication Type:Journal Article
Keywords: Idiopathic pulmonary hypertension (IPAH); MicroRNA-22; Biomarker; Myc binding protein (MYCBP)
From: Chinese Journal of Emergency Medicine 2012;21(1):57-60
CountryChina
Language:Chinese
Abstract: Objective To investigate the value of plasma miR-22 in diagnosis of idiopathic pulmonary arterial hypertension ( IPAH ),and its role of regulation mechanisms in the pathogenesis of the disease.Methods Circulating miR-22 levels of IPAH patients and healthy controls were evaluated by RTPCR.The silico analysis of targets for miR-22 was taken, and followed by eGFP reporter assay for verification of predicted target gene Myc binding protein (MYCBP). Results Compared with healthy controls,the expression of plasma miR-22 in IPAH patients was significantly decreased (P ＜ 0.01 ).The area under curve (AUC) of ROC curve was 0.744.MYCBP was a real target of miR-22 confirmed by silico analysis and eGFP reporter assay. Conclusions The expression of plasma miR-22 was significantly decreased in IPAH patients,and it could serve as a potential biomarker for diagnosis.The miR-22 might be involved in the pathogenesis of the disease through promoting its target gene MYCBP to activate the c-Myc pathway.