Proliferation, survival and differentiation of nerve stem cell in rat hippocampal after chronic stress
10.3760/cma.j.issn.1674-6554.2012.01.004
- VernacularTitle:慢性应激抑郁模型大鼠海马神经干细胞的增殖、存活和分化
- Author:
Weijuan QIAN
;
Yuxiu SUI
;
Yuan LI
- Publication Type:Journal Article
- Keywords:
Chronic unpredictable mild stress;
Neural stem cell;
Hippocampus
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2012;21(1):10-12
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of chronic stress on hippocampal neurogenesis.MethodsChronic unpredictable mild stress(CUMS) was used as animal model to provoke a decrease of neurogenesis in hippocampus.Simultaneously,hippocampal neurogenesis was monitored by assessing cell proliferation,survival,and differentiation.All rats were divided into control group,14 d CUMS group,28 d CUMS group.ResultsThe cell proliferation in 14d CUMS group(2254.17 ± 164.41 ) or 28d CUMS( 1900.33 ± 104.10) group were decreased contrast to the control (2919.50 ± 188.80) (P<0.01).The cell survival in 28 d CUMS group ( 1845.33± 126.88 ) were decreased contrast to the control (2404.50 ± 148.77 ) (P < 0.01 ).The cell differentiation had no difference between control group and CUMS group( NeuN/BrdU(71.63 ± 10.21 ) vs (69.11 ± 11.30),GFAP/BrdU ( 14.34 ± 2.03 ) vs ( 17.27 ± 2.93 ) ) (P > 0.05 ).ConclusionThese results suggest that chronic stress decreases cell proliferation and survival,but does not affect the phenotype of newborn cells in the hippocampus.