Therapeutic effects of replication-competent adenovirus carrying human endostatin gene on pancreatic cancer in mice
10.3760/cma.j.issn.1007-8118.2011.08.022
- VernacularTitle:携带人内皮抑素基因的增殖型腺病毒AdTPHre-hE对胰腺癌的体内实验研究
- Author:
Yifeng FANG
;
Yunfeng SHAN
;
Dingcun LUO
;
Qiyu ZHANG
- Publication Type:Journal Article
- Keywords:
Adenovirus;
Endostatin;
Pancreatic cancer
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(8):660-663
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish human pancreatic cancer xenografts in nude mice, and to investigate the antitumor efficacy of human endostatin expressed by replication-competent adenovirus AdTPHre-hE in vivo. Methods Pancreatic cancer cells AsPC-1 were injected subcutaneously in BALB/c nude mice to establish the xenografts. Tumor growth was observed and measured after AdTPHre-hE treatment. Expression of endostatin was detected by ELISA assay. The tumors were harvested for pathologic examination and immunohistochemical staining. Results Tumors grew more slowly in the AdTPHre-hE group and their sizes were markedly smaller than those of the Ad-hE group (P＜0.01)and control group(P＜0. 01). Endostatin levels were detected in the sera of nude mice in all treated groups, and endostatin expression in AdTPHre-hE group increased with time. The endostatin level in the AdTPHre-hE treated group was much higher(P＜0. 01)and increased faster than that in the Ad-hE treated group. Immunohistochemical staining for Hexon of adenovirus capsid showed more positive tumor cells in the tumor tissues treated with AdTPHre-hE. Immunohistochemical staining for FⅧ revealed a decreased microvessel density in the tumor tissues treated with AdTPHre-hE. Conclusion The replication-competent adenovirus efficiently expressed high-level endostatin and significantly inhibited tumor growth in vivo.
