Vasculogenic mimicry in human hepatocellular carcinoma
10.3760/cma.j.issn.1007-631X.2011.08.021
- VernacularTitle:人肝细胞癌血管生成拟态的实验研究
- Author:
Lunan QI
;
Liming SHANG
;
Tao PENG
;
Ya GUO
;
Kaiyin XIAO
;
Jiaquan LI
;
Lequn LI
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular;
Neovascularization,pathologic;
Gene,regulator
- From:
Chinese Journal of General Surgery
2011;26(8):687-690
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the existence of vasculogenic mimicry in hepatocellular carcinoma (HCC). Methods In this study 42 patients with a total of 47 HCC nodules underwent radical resection.Histological and immunohistochemical double staining of CD31 and PAS were applied to observe the existence of vasculogenic mimicry ( VM ). Reverse tanscription PCR (RT-PCR) were applied to study the expression of VE-cadherin, EPHA2 and MMP-2 genes. Results VM was found in 16 of the 42 (38. 1% )HCC cases. The typical forms of VM in the microscope are vessel-like structure formed by tumor cells,without endothelial cells and the PAS-positive looping pattern. The existence of VM in HCC correlates to a higher Edmondson grade, higher capacity of intrahepatic disseminating and poorer tumor-free survival time (P＜ 0. 05). Comparing the difference of VE-cadherin gene, EPHA2 gene and MMP-2 gene expression between VM positive nodes and in VM negative nodes by RT-PCR method demonstrated that VE-cadherin gene, EPHA2 gene and MMP-2 gene have a more intense expression in VM positive nodes than in VM negative nodes ( P ＜ 0. 05 ). Conclusion VM exists in human hepatocellular carcinoma. VM occurred more frequently in higher malignant HCC and predicts a higher rate of tumor recurrence and poorer prognosis.
