Role and mechanism of phosphate myosin light chain in chronic allograft nephropathy of rats
10.3760/cma.j.issn.0254-1785.2011.08.013
- VernacularTitle:磷酸化肌球蛋白轻链在大鼠慢性移植肾肾病早期病变中的作用及其机制
- Author:
Yuxin WANG
;
Yiqin ZHANG
;
Huaifu LI
;
Hequn ZOU
;
Yanling SHI
;
Ling CHEN
;
Wenying ZHOU
- Publication Type:Journal Article
- Keywords:
Rats;
Chronic allograft nephropathy;
Myosin light chains;
Integrin-linked kinase
- From:
Chinese Journal of Organ Transplantation
2011;32(8):497-501
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and mechanism of phosphate myosin light chain (pMLC) in the rat kidney of chronic allograft nephropathy (CAN) model. Methods The left donor kidneys from Fisher (F344) rats were orthotopically transplanted into Lewis recipients. Meanwhile, the F344 rats and LEW rats with resection of the right kidney served as control groups. Animals were harvested respectively at the 4th, 8th and 12th week after transplantation. The creatinine clearance rate (CCr) was calculated by urine creatinine of 24-h urine. Blood samples were collected from rats for determination of serum creatinine. The expression of pMLC was detected by using Western blotting and immunohistochernistry, and that of integrin-linked kinase (ILK) by using immunohistochemistry. Results Mononuclear cells infiltration of allografts was markedly aggravated as compared to the controls. Allografts got severe interstitial fibrosis and tubular atrophy at 12th week after transplantation. The expression of pMILC and ILK was up-regulated in the kidney of CAN rats after transplantation, and increased more significantly as the time went on. The expression of pMILC was significantly correlated with 24-h urine protein excretion (r= 0. 273, P＜0. 05), serum creatinine levels (r = 0. 434, P＜0. 01 ), the number of tubulointerstitial infiltrated mononuclear cells (r = 0. 525, P＜0. 01 ), the number of smooth muscle cells (SMC) in vascular wall (r= 0. 676, P＜0. 01 ) and the extent of interstitial fibrosis (r= 0. 570, P＜0. 01 ).There was a significantly positive correlation between ILK and pMLC in CAN rats at the 4th week after transplantation (r= 0. 778, P＜0. 01 ). Conclusion pMLC might play an key role in CAN, and the over-expression of ILK might be involve in the pathogenesis of CAN.
