A tumor transplant experiment: Theanines function to fight A549 with T lymph cells through stimulating dendritic cells (DC)
10.3760/cma.j.issn.1008-1372.2011.07.007
- VernacularTitle:茶氨酸刺激树突状细胞调节T淋巴细胞抑制抗肺癌A549细胞移植瘤实验研究
- Author:
Mingsheng LEI
;
Min LI
;
Qihua GU
;
Chengping HU
- Publication Type:Journal Article
- Keywords:
Amino acids/PD;
Dendritic cells/DE;
T-lymphocytes/DE;
Lung neoplasms/IM;
Lung/CY
- From:
Journal of Chinese Physician
2011;13(7):887-891
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reveal the impact of anti-lung cancer A549 by theanine, which may regulate T lymphocytes through dendritic cells (DCs), and to observe the immune protection of theanine on SCID mice transplanted tumors. Methods SCID mice were reconstructed after immunization, then A549 cells were inoculated. The immune protection and immune treatment of theanine stimulated DCs were observed. Results In the theanine-stimulated DC group, as well as DC group, the time needed to form tumor were significantly prolonged (F = 29. 5, P ＜ 0. 01). And the transplanted tumors were smallest (F =69. 51, P ＜0. 01) and lightest (F = 190. 9, P ＜0. 01) in the theanine-stimulated DC group. 50 days after tumor-bearing, the transplanted tumors in the theanine-stimulated DC group were smaller than in the control group, and the surface were smoother with no adhesions. Under light microscope, a large thin slice necrosis was showed in the theanine-stimulated DC group, and the VEGF expression was also reduced (F = 31.4, P ＜ 0. 01). Conclusions The anti-tumor immune protection and treatment mechanism of theanine-stimulated DCs were possibly through regulation of T cells, as well as the VEGF expression reducing,thereby inhibited tumor blood vessel formation.
