The proapoptotic effect of combined treatment with human recombinant TNF-related apoptosis-inducing ligand and Celecoxib on gallbladder carcinoma
10.3760/cma.j.issn.1007-8118.2011.08.023
- VernacularTitle:人重组TNF相关凋亡诱导配体联合塞来昔布诱导胆囊癌细胞凋亡的作用研究
- Author:
Huajie ZONG
;
Baobing YIN
;
Jinhong CHENG
;
Baojin MA
;
Duan CAI
- Publication Type:Journal Article
- Keywords:
TNF related apoptosis inducing ligand;
Celecoxib;
Gallbladder carcinoma;
Apoptosis
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(8):664-668
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of combined treatment with rhTRAIL(recombinant human TNF-related apoptosis inducing ligand) and selective Cox-2 inhibitor Celecoxib on gallbladder carcinoma in vitro and to explore the possible mechanism of the effect. Methods Western blot analysis was used to detect the expression of c-FLIP and death receptors after treatment by Celecoxib. Apoptosis of gallbladder cell line SGC-996 after the combined treatment with Celecoxib and rhTRAIL was detected in three ways: (1) phase microscopy of the cells, (2) detection of effector caspase-3 and caspase-7 activity, and (3) determination of the proportion of apoptotic cells labeled by Annexin V-PI flow cytometric analysis using CELLQUEST software. Results Celecoxib down-regulated the expression of c-FLIPs and up-regulated the expression of DR5 in a dose- and time-dependent mode on cell line SGC-996. Apoptotic levels in the combined treatment group in cell line SGC-996 were significantly higher than those in the single drug treatment group and control group. Conclusion Celecoxib markedly sensitized rhTRAIL-induced apoptosis through the down-regulation of c-FLIPs and up-regulation of DR5 in gallbladder carcinoma cell line SGC-996.
