Apoptosis and oxidative injury of donor islets during isolation and purification

VernacularTitle:人胰岛在分离、纯化过程中的细胞凋亡及氧化损伤
Author: Xuyong SUN ; Ke QIN ; Jiang NONG ; Ning WEN ; Yanhua LAI ; Jianhui DONG ; Feng NIE ; Wene CAI ; Yinhong QIN ; Chen HUANG
Publication Type:Journal Article
Keywords: Islet; Transplantation; Apoptosis; Injury of oxidation
From: Chinese Journal of Organ Transplantation 2011;32(8):502-505
CountryChina
Language:Chinese
Abstract: Objective To observe the changes of islet cell apoptosis and oxidation-antioxidation before the transplantation, and to explore the pathways of islet protection. Methods Fifteen human pancreases were perfused with the Hanks solution containing collagenase, then digested and isolated. During the procedure, islet cell apoptosis was detected by TUNEL, SOD and MDA in the pancreas were measured by colorimetric method, and the morphologic changes were observed by H-E staining and dithizone staining. Results In the procedure of human islet isolation, especially in the stage of digestion, the apoptosis of human islet cells occurred. In the stages of perfusion and digestion, the MDA contents reached the high levels (6. 18 ± 2. 38 and 9. 21 ± 2. 75 umol/mg protein respectively),and the structures of the islets and tissues around the islets were damaged. Conclusion In the stages of perfusion and digestion, apoptosis of islet cells can be caused by oxidation. It suggests that antioxidation is a pathway for protection of islets before transplantation.