Effects of hypertriglyceridemia on NO, ET-1 and the grade of the pathology severity in rats with severe acute pancreatitis
10.3760/cma.j.issn.1008-1372.2011.07.010
- VernacularTitle:甘油三酯对大鼠重症胰腺炎内皮素和一氧化氮表达及病理评分的影响
- Author:
Xiaochen ZENG
;
Xiangying WANG
- Publication Type:Journal Article
- Keywords:
Triglycerides/AE;
Pancreatitis/ME/PA;
Endothelins/ME;
Nitric oxide/ME
- From:
Journal of Chinese Physician
2011;13(7):900-902
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore influence of hypertriglyceridemia on serum NO ET-1 and the grade of the pathology severity in rats with severe acute pancreatitis. Methods 36 SD rats were randomly divided into 2 groups, group A (HLSAP group) , group B (SAP group). Severe acute pancreatitis was constructed by retrograde injection of 5% na-taurocholate. Blood samples were taken from all subjects to measure triglyceride, ET-1 and NO, pancreatic tissue samples were taken from head of pancreas and stained with H. E. , the degree of pancreatic damage was observed according to the point score of Schmidt and Pozsar's methods. Results In group A, the concentration of ET-1 increased more obviously than that in group B at 4 hour and 8 hour period(P ＜0. 05). The concentration of NO declined both in group A and group B at 12 hours period,but it had a great decline in group A. Animals with hyperlipidemia and severe acute pancreatitis developed significantly higher(P ＜0. 05) ET-1 than the animals of the non-hyperlipamic severe acute pancreatit group in 4 hours and 8 hours period. NO declined in group A and group B at 12 hours period, group A have significantly higher(P ＜0. 05) decline than group B in NO. The histological degree of pancreatic damage were significantly higher in group A than that in the group B at all times. Conclusions Mircrocirculation disorder had existed disorder in the early of SAP. Hypertriglyceridemia could incrase ET-1 and NO earlier and higher in severe acute pancreatitis, and then decline in the late stage. Hypertriglyceridemia intensified the pathologichistological degree of pancreatic damage.
