Regulation of osteoclast inhibitory lectin ( OCIL ) expression by prostaglandin E2 in rat osteoblastic cells
10.3760/cma.j.issn.1000-6699.2011.08.019
- VernacularTitle:前列腺素E2调节大鼠成骨细胞OCIL基因表达的信号通路研究
- Author:
Jinxing QUAN
;
Baoli WANG
;
Fang ZHENG
;
Mingcai QIU
- Publication Type:Journal Article
- Keywords:
prostaglandin E2;
Osteoclast inhibitory lectin;
Signaling pathway;
Osteoblasts
- From:
Chinese Journal of Endocrinology and Metabolism
2011;27(8):683-686
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the regulation of osteoclast inhibitory lectin (OCIL) mRNA expression by prostaglandin E2 ( PGE2 ) in rat osteoblastic cells and the involved signaling pathways. Rat primary osteoblasts and UMR106 osteoblast-like cells were cultured and treated with various doses of PGE2 or regulators of different signaling pathways for different periods of time, the cells were then harvested at indicated dates. Total RNA were isolated and OCIL mRNA expression were studied by real-time PCR. PGE2, Forskolin, db-cAMP, and A23187 increased OCIL mRNA by 2. 38 fold,4. 2 fold,4. 5 fold, and 5. 1 fold ( all P＜0. 01 ) respectively, while PMA downregulated OCIL mRNA expression by 50% ( P＜0. 01 ). KT-5720, verapamil, W7, and PD98059 downregulated PGE2 induced OCIL mRNA expression by 56%, 40%, 65%, and 60%, respectively( all P＜0. 0l ). While chelerythrine enhanced PGE2 induced OCIL mRNA expression by 30% ( P＜0. 05 ). PGE2 up-regulated the expression of OCIL in rat osteoblastic cells via PKA, MAPK, and Ca2+/Calmodulin signaling pathways.
