Assessment of T cell subsets and sjTREC gene level in the sepsis children
10.3760/cma.j.issn.1673-4912.2011.04.008
- VernacularTitle:脓毒症患儿T细胞亚群和sjTREC基因水平的评估
- Author:
Qing CAO
;
Kang AN
;
Jian ZHANG
;
Bihu LI
- Publication Type:Journal Article
- Keywords:
Sepsis;
T-lymphocyte subsets;
Thymic output function;
sjTREC gene
- From:
Chinese Pediatric Emergency Medicine
2011;18(4):312-315
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate T-lymphocyte subsets and sjTREC gene level in sepsis children and to provide a reasonable theoretical basis for immune regulation. Methods This prospective study was performed on children who were classified as sepsis group (n = 25), severe sepsis group (n = 23), and control group (n = 50). The T cell subsets were measured before the blood products,immune agents,and nutritional support were administrated. By real-time fluorescence quantitive-PCR method, the sjTREC levels of the patients with sepsis and healthy children were quantitatively detected respectively;then the sjTREC levels were absolutely quantified by two standard curve method. The results were demonstrated by sjTREC and endogenous reference gene (Cα) copies ratio (sjTREC/Cα× 2 × 1000) and statistically analyzed by SPSS 16. 0 software. Results The levels of CD3 +, CD4 +, and CD8 + T cells in severe sepsis group were lower than those in control group (P＜0.01). The level of sjTREC of severe sepsis group was 173.86 +38.37,which was significantly lower than those of sepsis group (345. 15 ± 66. 21) and control group (389. 42 ± 50. 20) (P ＜ 0. 01). Conclusion Children with severe sepsis have a range of T-lymphocyte subsets disorders and decreased thymic output function, so early immunotherapy can improve clinical outcome.
