Effects of isoflurane anenthesia on myocyte enhancer factor 2 signaling pathway in neonatal rat hippocampus
10.3760/cma.j.issn.0254-1416.2011.06.018
- VernacularTitle:异氟醚麻醉对新生大鼠海马激活肌细胞增强因子2信号通路的影响
- Author:
Yilin ZHAO
;
Ailin LUO
;
Xiaogao JIN
;
Jintao WANG
;
Lei TAN
;
Qingyu SHI
;
Shiyong LI
- Publication Type:Journal Article
- Keywords:
Isoflurane;
Myogenic regulatory factors;
Infant,newborn;
Hippocampus
- From:
Chinese Journal of Anesthesiology
2011;31(6):714-716
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of isoflurane anenthesia on myocyte enhancer factor 2(MEF2) signaling pathway in neonatal rat hippocampus. Methods Twenty-four 5-day-old SD rats of both sexes,weighing 10-13 g, were randomly divided into 2 groups ( n = 12 each): control group (group C) and isoflurane group (group I). In group I, 1.5% isoflurane in 100% O2 was inhaled for 6 h. Group C received no treatment.Three rata in each group were sacrificed at 2, 4, 6 h of isoflurane anenthesia and 24 h after isoflurane anenthesia (T1-4), and the hippocampi removed for determination of MEF2 mRNA, synGAP Ⅰ mRNA, Arc mRNA and synapsinⅠ mRNA expression (by PT-PCR) and synapsin Ⅰ protein expression (by Western blot).Results Compared with group C, the expression of MEF2 mRNA, synGAP Ⅰ mRNA, Arc mRNA and synapsin Ⅰ mRNA at T1-3 and synapsin Ⅰ protein at T2-4 was up-regulated in group I ( P ＜ 0.05). Conclusion Inhalation of anaesthetic concentration of isoflurane may affect synapse formation during the development of central nervous system by actirating hippocampal MEF2 signaling pathways in neonatal rats.
