Effects of intestinal ischemia-reperfusion on brain in rats
10.3760/cma.j.issn.0254-1416.2011.06.026
- VernacularTitle:肠缺血再灌注对大鼠脑的影响
- Author:
Jun ZHOU
;
Wenqi HUANG
;
Cai LI
;
Guiyun WU
;
Yunsheng LI
;
Shihong WEN
;
Wanlong LEI
;
Kexuan LIU
- Publication Type:Journal Article
- Keywords:
Intestine;
Repeffusion injury;
Brain;
Cognition
- From:
Chinese Journal of Anesthesiology
2011;31(6):739-742
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of intestinal ischemia-reperfusion (I/R) on the brain in rats. Methods Sixty-four healthy male SD rats weighing 250-300 g were randomly allocated to one of 2 groups (n = 32 each): sham operation group (S) and intestinal I/R group (I/R). Intestinal I/R was produced by occlusion of superior mesenteric artery (SMA) for 90 min followed by reperfusion. Eight animals were sacrificed at each of the following time points: 2, 6, 12 and 24 h of reperfusion (T1-4) in each group. After a median sternotomyblood samples were taken from left ventricle for measurement of plasma TNF-α and IL-6 (by ELISA). Intestine and brain tissue was harvested for microscopic examination and detection of apoptosis ( by TUNEL). The cognitive function was tested using Morris water maze at 24 h. Results No abnormality was found in intestine and brain tissue in group S. Intestinal damage and neurodegeneration were detected in group I/R. Intestinal I/R significantly increased cerebral apoptosis in group I/R compared with group S. Plasma TNF-a and IL-6 concentrations were significantly higher at T1-4 in group I/R than in group S. The escape latency and swimming distance were significantly increased, while the number of crossing the platform was decreased in group I/R compared with group S. There was no significant difference in the swimming speed between the 2 groups. Conclusion Intestinal I/R can induce brain injury and lead to cognitive dysfunction. I/R-induced release of inflammatory mediators and neuronal apoptosis are involved in the underlying mechanism.
