Effect of propofol pretreatment on hippocampal monocyte chemotactic protein-1 and CC-chemokine receptor type 2 expression following forebrain ischemia-reperfusion in rats
10.3760/cma.j.issn.0254-1416.2011.06.030
- VernacularTitle:异丙酚预先给药对大鼠前脑缺血再灌注时海马单核细胞趋化因子1及其受体表达的影响
- Author:
Yongqing GUO
;
Xiaolai HOU
;
Youzhang LIU
;
Huaping ZHANG
;
Zheng GUO
- Publication Type:Journal Article
- Keywords:
Propofol;
Chemokine CCL2;
Receptors,CCR2;
Reperfasion injury;
Brain
- From:
Chinese Journal of Anesthesiology
2011;31(6):754-756
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of propofol pretreatment on hippocampal monocyte chemotactic protein-1 ( MCP-1 ) and CC-chemokine receptor type 2 (CCR2) expression following forebrain ischemiarepcrfusion (I/R) in rats. Methods Twenty-four male SD rats weighing 250-300 g were randomly divided into 3 groups ( n = 8 each): group Ⅰ control; group Ⅱ I/R and group Ⅲ propofol pretreatment. Cerebral I/R was induced by clamping bilateral common carotid arteries for 10 min combined with hypotension ( MAP was maintained at 35-45 mm Hg) induced by exsanguinations in group Ⅱ and Ⅲ. In group Ⅲ propofol 50 mg/kg was injected into femoral vein immediately before cerebral ischemia. The animals were sacrificed at 6 h of reperfusion. Hippocampal tissue was obtained for detection of MCP-1 mRNA and CCR2 mRNA and their protein expression by RT-PCR and Western blot technique. Results I/R significantly increased the expression of MCP-1 and CCR2 in hippoeampal tissue as compared with control group. Propofol pretreatment significantly attenuated cerebral I/R induced increase in MCP-1 and CCR2 expression. Conclusion Propofol pretreatment can significantly inhibit forebrain I/R-induced hippocampal MCP-1 and CCP2 expression.
