Differential expression profile in the progeny of normal human liver cells irradiated by 60Co γ-rays
10.3760/cma.j.issn.0254-5098.2011.04.012
- VernacularTitle:60Co γ射线照射后人正常肝细胞子代的基因差异表达
- Author:
Yahui ZUO
;
Xuhong DANG
;
Fang WANG
;
Xiaoli WANG
;
Zhongwen WANG
;
Jian TONG
- Publication Type:Journal Article
- Keywords:
Genechip;
Genes differential expression;
Ionizing radiation;
Genomic instability
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(4):425-429
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differential expression profile in the progeny of human liver cells surviving from ionizing radiation.Methods Complemental DNA gene chip was used to measure the transcriptional profile in progeny of HL-7702 cells exposed to 0, 2, 4, and 6 Gy of 60Co γ-rays, and the differentially expressed genes HAVCR2 and RAN were further identified by real-time PCR.Results The transcription level of 262 genes, 2746 genes and 3406 genes changed in the progeny of survival cells at 2, 4 and 6 Gy, respectively.A total of 71 common differentially expressed genes were screened, most of which were associated with transduction, cell cycle regulation, cellular immunity, cytoskeleton and movement, cell replication and repair mechanism.Conclusions Ionizing radiation could induce the expression changes of many genes, which might reveal the molecular mechanisms of gene expression in radiation induced genomic instability.
