Enhancement of distribution of dermal multipotent stem cells to bone marrow in rats of total body irradiation by platelet-derived growth factor-AA treatment
10.3760/cma.j.issn.0254-5098.2011.04.014
- VernacularTitle:血小板源性生长因子促进移植真皮多能干细胞向全身照射大鼠骨髓的分布
- Author:
Zhaowen ZONG
;
Yongchuan REN
;
Yue SHEN
;
Yonghua CHEN
;
Xinze RAN
;
Chunmeng SHI
;
Tianmin CHENG
- Publication Type:Journal Article
- Keywords:
Radiation injury;
Dermal multipotent stem cell;
Platelet-derived growth factor-AA
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(4):433-436
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether dermal multipotent stem cells (dMSCs) treated with platelet-derived growth factor-AA ( PDGF-AA )could distribute more frequently to the bone marrow in rats of total body irradiation (TBI).Methods Male dMSCs were isolated and 10 μg/L PDGF-AA was added to the culture medium and further cultured for 2 h.Then the expression of tenascin-C were examined by Western blot, and the migration ability of dMSCs was assessed in transwell chamber.The pre-treated dMSCs were transplanted by tail vein injection into female rats administered with total body irradiation, and 2 weeks after transplantation, real-time PCR was employed to measure the amount of dMSCs in bone marrow.Non-treated dMSCs served as control.Results PDGF-AA treatment increased the expression of tenascin-C in dMSCs, made (1.79 ± 0.13) × 105 cells migrate to the lower chamber under the effect of bone marrow extract, and distributed to bone marrow in TBI rats, significantly more than ( 1.24 ± 0.09) ×105 in non-treated dMSCs (t = 8.833, P ＜ 0.0l ).Conclusions PDGF-AA treatment could enhance the migration ability of dMSCs and increase the amount of dMSCs in bone marrow of TBI rats after transplantation.
