Effect of liraglutide on expressions of plasminogen activator inhibitor 1 and intercellular adhesion molecule 1 in cultured rat glomerular mesangial cells stimulated by TNF-α

VernacularTitle:利拉鲁肽对肿瘤坏死因子α刺激下大鼠肾小球系膜细胞纤溶酶原激活物抑制物1和胞间黏附分子1表达的影响
Author: Fandong MENG ; Wei LI
Publication Type:Journal Article
Keywords: Diabetic nephropathies; Tumor necrosis factor-alpha; Plasminogen activator inhibitor 1; Intercellular adhesion molecule-1; Liraglutide
From: Chinese Journal of Nephrology 2012;28(9):715-719
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of linraglutide on expressions of plasminogen activator inhibitor 1 (PAI-1) and intercellular adhesion molecule 1 (ICAM-1) in cultured rat glomerular mesangial cells (RGMCs) stimulated by tumor necrosis factor α (TNF-α).Methods Cultured HBZY21 RGMCs were divided into 6 groups:normal control cells,TNF-α stimulated cells,liraglutide low (10 nmol/L),median (100 nmol/L) and high (1000 nmol/L) concentration incubated cells stimulated with TNF-α,PDTC intervention cells.The expressions of PAI-1 and ICAM-1 of each group were measured by ELISA and RT-PCR.Results The levels of PAI-1 and ICAM-1 protein and mRNA were remarkably increased by TNF-α (P＜0.05),and liraglutide could inhibit above up-regulated expressions (all P＜0.05).Compared with TNF-α-stimulated group,the expressions of PAI-1 and ICAM-1 were decreased by PDTC intervention (all P＜0.05).Conclusion Liraglutide can partly down-regulate the expressions of PAI-1 and ICAM-1 induced by TNF-α in RGMCs.