The study of ischemia postconditioning intervention in a rabbit's acute mesenteric ischemia-reperfusion injury model
10.3760/cma.j.issn.1007-631X.2012.09.015
- VernacularTitle:缺血后适应在兔急性肠系膜缺血再灌注损伤模型中的作用
- Author:
Mou YANG
;
Juwen ZHANG
;
Ping CHEN
;
Lin SUN
;
Haijie CHE
;
Jun YONG
;
Lubin LI
;
Fubo SONG
- Publication Type:Journal Article
- Keywords:
Mesenteric vascular occlusion;
Ischemia;
Reperfusion injury;
Disease models,animal;
Animals experimentation
- From:
Chinese Journal of General Surgery
2012;27(9):740-742
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the effect of ischemia postconditioning intervention in a rabbit's acute mesenteric ischemia-reperfusion injury model.Methods 120 rabbits were divided randomly into Con( only expose SMA by operation),I/R( clamping SMA 30 min,reperfusing 120 min),IpostC1 ( clamping SMA 30 min,3 clamping 30 s/releasing 30 s round,reperfusing 117 min),and IpostC2 (clamping SMA 30 min,3 clamping 60 s/releasing 60 s round,reperfusing 114 min) group (n =30).Levels of MDA and MPO in serum and intestinal tissues were measured. Chiu-6 standard scoring was used to determine the pathology score of injured intestinal mucosae.ResultsCompared with the Con group,MDA and MPO levels in serum and intestinal tissues increased obviously in the three other groups,the same as in the pathology score of injured intestinal mucosae (P ＜ 0.01 ) ; Compared with the I/R group,the MDA and MPO levels in serum and intestinal tissues decreased obviously in the IpostC1 group ( P ＜ 0.01 ),but not in the IpostC2 group ( P ＞ 0.05 ).ConclusionsMDA and MPO levels in serum and intestinal tissues and intestinal mucosal injury decreased obviously in the rabbit's acute mesenteric ischemia-reperfusion injury model by ischemia postconditioning intervention.
