Expression and Purification of Enterovirus Type 71 Polyprotein P1 using Pichia pastoris system
10.1007/sl2250-012-3256-7
- Author:
Xue HAN
;
Xiaoling YING
;
Hao HUANG
;
Shili ZHOU
;
Qi HUANG
- Publication Type:Journal Article
- Keywords:
EV71-P1 protein;
Expression;
Purification;
Immunogenicity;
Pichia pastoris
- From:
Virologica Sinica
2012;27(4):254-258
- CountryChina
- Language:Chinese
-
Abstract:
Enterovirus type 71(EV71) causes severe hand-foot-and-mouth disease(HFMD) resulting in hundreds of deaths of children every year; However,currently,there is no effective treatment for EV71.In this study,the EV71 poly-protein(EV71-P1 protein) gene was processed and cloned into the eukaryotic expression vector pPIC9k and then expressed in Pichia pastoris strain GS115.The EV71 P1 pretein with a molecular weight of 100 kD was produced and secreted into the medium.The soluble EV71 P1 protein was purified by column chromatography with a recovery efficiency of 70％.The result of the immunological analysis showed that the EV71 P1 protein had excellent immunogenicity and could stimulate the production of EV71-VP1 IgG antibody in injected rabbits.We suggest that EV71-P1 protein is an ideal candidate for an EV71 vaccine to prevent EV71 infection.
