Effects of Geraniin on Platelet Aggregation and Interactions between Platelets and Neutrophils
10.3969/j.issn.1003-4706.2012.05.002
- VernacularTitle:老鹳草素对血小板聚集和血小板-中性粒细胞之间相互作用的影响
- Author:
Peng CHEN
;
Fan LI
;
Bo HE
;
Hongxiang WU
;
Jianyu YANG
;
Xiaochao ZHANG
;
Zhiqiang SHEN
- Publication Type:Journal Article
- Keywords:
Geraniin;
Platelet aggregation;
Calcium;
Platelet-neutrophil interactions
- From:
Journal of Kunming Medical University
2012;33(5):4-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of geraniin on platelet aggregation and platelet-neutrophil interactions.Methods Platelet aggregation,in vitro and ex vivo,was determined by use of Born's method,and the binding of thrombin-stimulated platelets to neutrophils was observed based on the rosette assay.Intracellular calcium concentration of platelets was measured by using Fura-2-AM.Results Geraniin in vitro significantly inhibited arachidonic acid (AA)-,adenosine diphosphate (ADP)-,or platelet activating factor (PAF)-induced platelet aggregation,in a concentration-dependent manner.The medium inhibitory concentrations (IC50) were 2.4,0.4 and 1.1 μmol/L,respectively.Intragastric geraniin at 5 mg/kg markedly suppressed platelet aggregation induced by AA,ADP,or PAF.Geraniin decreased the total rise of [Ca2+]i,Ca2+ release,and Ca2+ influx,in a concentration-dependant manner.The IC50 values were 71.9,84.9,and 62.9 μmol/L,respectively.Geraniin decreased the binding of thrombin-stimulated platelets to neutrophils,and significantly inhibited washed platelet aggregation stimulated by fMLP-activated neutrophils.The IC50 values were 3.2 and 10.2 μmol/L,respectively.Conclusion It is suggested that geraniin inhibited platelet aggregation in vitro and ex vivo,decreased the calcium mobilization of platelets,and suppressed the interactions between platelets and neutrophils.
