Preparation and Identification of Anti-rabies Virus Monoclonal Antibodies
10.1007/s12250-012-3242-0
- Author:
Wenjuan WANG
;
Xiong LI
;
Lihua WANG
;
Hu SHAN
;
Lei CAO
;
Pengcheng YU
;
Qing TANG
;
Guodong LIANG
- Publication Type:Journal Article
- Keywords:
Rabies virus;
Monoclonal antibodies;
Specificity;
Detection
- From:
Virologica Sinica
2012;27(3):172-178
- CountryChina
- Language:Chinese
-
Abstract:
To provide a foundation for the development of rapid and specific methods for the diagnosis of rabies virus infection,anti-rabies virus monoclonal antibodies were prepared and rabies virus nucleoprotein and human rabies virus vaccine strain (PV strain) were used as immunogens to immunize 6-8 week old female BALB/c mice.Spleen cells and SP2/0 myeloma cells were fused according to conventional methods:the monoclonal cell strains obtained were selected using the indirect immunofluorescence test; this was followed by preparation of monoclonal antibody ascitic fluid; and finally,systematic identification of subclass,specificity and sensitivity was carried out.Two high potency and specific monoclonal antibodies against rabies virus were obtained and named 3B12 and 4A12,with ascitic fluid titers of 1∶8000 and 1∶10000,respectively.Both belonged to the IgG2a subclass.These strains secrete potent,stable and specific anti-rabies virus monoclonal antibodies,which makes them well suited for the development of rabies diagnosis reagents.
