Role of N-cadherin in cranial neural crest delamination during chick embryogenesis
10.3969/j.issn.1000-4718.2012.07.022
- VernacularTitle:鸡胚发育中神经钙黏蛋白对头部神经嵴细胞分层的作用
- Author:
Haomin WEN
;
Yan LI
;
Guang WANG
;
Xiaotan ZHANG
;
Xiaoyu WANG
;
Zhaolong ZHANG
;
Xuesong YANG
- Publication Type:Journal Article
- Keywords:
Neural crest;
N-cadherin;
Migration;
Delamination;
Chick embryo
- From:
Chinese Journal of Pathophysiology
2012;28(7):1269-1274
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of N-cadherin in the delamination of neural crest cells. METHODS: The normal expression of N-cadherin in neural tube was identified using in situ hybridization. The cells with N-cadherin over expression were obtained by transfection of wild-type N-cadherin (wt-N-cadherin) ,and the cells with N-cadherin silencing expression were obtained by transfection of dominant-negative N-cadherin (dn-N-cadherin). The migration of cranial neural crest cells was determined by the technique of immunohistochemistry. RESULTS: Either overexpression or down-regulation of N-cadherin significantly affected the migration of cranial neural crest cells. CONCLUSION: Delamination and migration of the cranial neural crest cells rely on the relative N-cadherin expression in the neural tube during neurulation.
