A novel 5' splice site mutation in ACTC1 gene may play an important role in congenital ventricular septal defect
10.3969/j.issn.1673-8225.2012.11.044
- VernacularTitle:一个新的ACTC1基因5'端剪切位点突变可能在先天性心脏病室间隔缺损发病中起重要作用
- Author:
Hang LI
;
Binbin WANG
;
Bingren GAO
;
Jiangyan LIU
;
Qiming ZHAO
;
Wensheng CHEN
;
Wei WANG
;
Kun YANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2012;16(11):2087-2090
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: As a candidate gene of congenital heart disease, ACTC1 gene is related to congenital atrial septal defect inhumans.OBJECTIVE: To perform a mutation screen of ACTC1 gene in 110 nuclear families of congenital heart disease.METHODS: A case-control study was conducted based on 110 nuclear families of congenital heart disease and 300 normalhuman beings with no reported cardiac malformation. Six fragments in the coding region of ACTC1 gene was amplified by PCR invitro using five primers pairs. PCR products were screened for gene mutations.RESULTS AND CONCLUSION: A novel G-to-A variant was found at the third nucleotide of the intron downstream from exon 5.This mutation existed in a 5-year-old female with an isolated ventricular septal defect and her 30-year-old father, who had noreported cardiac anomalies. This mutation was not detected in 300 normal controls. These findings indicate that the mutation maybe related with congenital ventricular septal defects in humans.
