N-acetylcysteine protects bone marrow stromal cells against the toxicity of 6-hydroxydopamine

VernacularTitle:N-乙酰半胱氨酸拮抗6-羟基多巴胺对骨髓基质细胞的毒性作用
Author: Qilin ZHANG ; Weifeng LUO ; Henghui WANG ; Yan YE ; Tingge ZHU ; Chunfeng LIU
Publication Type:Journal Article
From: Chinese Journal of Tissue Engineering Research 2012;16(6):985-988
CountryChina
Language:Chinese
Abstract: BACKGROUND: 6-hydroxydopamine, as an endogenous toxic factor in the pathogenesis of Parkinson's disease, participates in oxidative stress. N-acetylcysteine resists oxidation and removes free radicals effectively.OBJECTIVE: To investigate the toxicity of 6-hydroxydopamine in bone marrow stromal cells and the antagonistic effect of N-acetylcysteine on it. METHODS: Bone marrow stromal cells of Sprague-Dawley rats were cultured in vitro. Bone marrow stromal cells of passage 3 were treated with 6-hydroxydopamine with the final concentrations of 0,0.05,0.1g/L and N-acetylcysteine with the final concentrations of 0, 0.075,0.3,1.2,4.8g/L, respectively.RESULTS AND CONCLUSION: MTT assay showed that 6-hydroxydopamine (0.05 and 0.1 g/L) significantly decreased the viability of bone marrow stromal cells. This toxic effect of 6-hydroxydopamine was significantly inhibited by 0.3 g/L N-acetylcysteine. It suggests that antioxidant N-acetylcysteine may affect the toxic action of 6-hydroxydopamine.