Inhibitory Effects of Ginsenoside Rb1 on Apoptosis Caused by HSV-1 in Human Glioma Cells
10.1007/s12250-012-3220-6
- Author:
Yuanyuan LIANG
;
Bin WANG
;
Dongmeng QIAN
;
Ling LI
;
Zhihao WANG
;
Ming HU
;
Xuxia SONG
- Publication Type:Journal Article
- Keywords:
Ginsenoside Rb 1;
Herpes Simplex Virus- 1;
Human Glioma Cells;
Apoptosis
- From:
Virologica Sinica
2012;27(1):19-25
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the inhibitory effects of Ginsenoside Rb1 (GRb1) on apoptosis caused by Herpes Simplex Virus-1 (HSV-1) in Human Glioma Cells (U251),U251 cells were infected by HSV-1 at a multiplicity of infection of 5 and GRb1,GRb1+HSV-1,HSV-1 and control groups.MTT and cell apoptosis assays were used to detect the inhibitory effects of GRbl on the apoptosis of U251 cells that caused by HSV-1 infection for various concentrations of drug and virus treatments by MTT assay.We found that in the 400 μg/mL GRbl and 400 μg/mL GRbl+HSV-1 groups,MTT values were higher than control group at all times (P＜0.05).Moreover,the apoptosis rate in the 400 μg/mL GRb1+HSV-1 group was lower than the HSV-1 group (P＜0.05).These results confirmed that,at appropriate concentrations,GRb 1 could inhibit nerve cell apoptosis in HSV-1 infections.
