Effect of cocaine-amphetamine-regulated transcript peptide on the content of 4-hydroxy-2-noneral and infarct volume after cerebral ischenia/reperfusion in mice
10.3760/cma.i.issn.1673-4165.2012.02.006
- VernacularTitle:可卡因-苯丙胺调节转录肽对脑缺血再灌注小鼠梗死体积和脑组织4-羟壬烯醛含量的影响
- Author:
Zhenhan ZHU
;
Dujuan SHA
;
Qiming LI
;
Jin LI
;
Yong HAN
;
Shuangshuang GU
;
Jun ZHANG
- Publication Type:Journal Article
- Keywords:
Cocaine- And Amphetamine-Regulated Transcript Protein;
Brain Ischemia;
Stroke;
4-Hydroxy-2-Nonenal;
Neuroprotective Agents;
Mice
- From:
International Journal of Cerebrovascular Diseases
2012;20(2):142-146
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cocaine-amphetamine-regulated transcript peptide (CART) on the content of 4-hydroxy-2-noneral (HNE) and infarct volume after cerebral ischemia/reperfusion in mice.Methods A total of 96 healthy male mice were randomly divided into four groups:ischemia/reperfusion (n =27),CART (n =27),normal saline control (n =27) and sham operation (n =15) groups.A middle cerebral artery occlusion (MCAO) model was induced.Two hours after MCAO,CART 55-102 and equivalent normal saline were injected respectively via the tail veins of mice in the CART group and the normal saline control group,and then they were injected every other 24 hour.The neurological scores,infarct volume and the HNE content of lipid metabolism of oxidative stress were performed and detected respectively at 12,24,48 and 72hours after reperfusion.Results CART could significantly improve the neurological deficit scores (all P ＜0.05) and reduce infarct volume (all P＜0.05) at different time points after ischemia/reperfusion.The content of HNE was upregulated (all P＜0.05) at different points after referfusion.CART could significantly down-regulate the increased HNE levd in brain after ischemia (all P＜0.05).Conclusions CART may protect ischemic brain injury in mice by inhibiting lipid peroxidation.
