Application of label-free quantitative proteomics in keloid

VernacularTitle:非标记定量技术研究瘢痕疙瘩的蛋白质组学特点
Author: Liqun YANG ; Xiaodong CHEN ; Xiaodong YAO
Publication Type:Journal Article
Keywords: Keloid; Proteomics; Chromatography,high pressure liquid; Tandem mass spectrometry
From: Chinese Journal of Dermatology 2012;45(3):173-177
CountryChina
Language:Chinese
Abstract: Objective To identify differentially expressed proteins in keloid tissue and to explore the proteomic characteristics of keloid by utilizing capillary high-performance liquid chromatography (LC) and tandem mass spectrometry (MS).Methods Tissue specimens were obtained from the lesions of 5 patients with non-familial keloid and normal skin of 5 human controls.After extraction and in-solution digestion of proteins,the resultant peptides were separated and identified by LC-MS/MS.Label-free detection and quantitation of peptides were carried out by using the DeCyder MSTM software.Results A total of 570differentially expressed peptides were identified between non-familial keloid and normal tissue (all P ＜ 0.05),which correspond to 1221 proteins.A difference greater than 1.5 folds was observed in the expressions of 293 proteins,including 124 up-regulated and 169 down-regulated proteins in non-familial keloid tissue.Conclusion Differences exist in the expression of proteins between non-familial keloid and normal skin tissue.