Molecular mechanism of dexamethasone affecting podocyte motility
10.3760/cma.j.issn.1001-7097.2012.09.009
- VernacularTitle:地塞米松影响足细胞活动力的相关分子研究
- Author:
Haimei LIU
;
Hong XU
;
Xia GAO
;
Yibing ZHENG
;
Junchao LIU
;
Xiliang ZHA
- Publication Type:Journal Article
- Keywords:
Dexamethasone;
Podocytes;
Actinin;
Cell movement;
Nephrin
- From:
Chinese Journal of Nephrology
2012;28(9):705-709
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential roles of dexamethasone (Dex) in podocyte motility,and to explore the mechanism of modulating α-actinin-4,nephrin.Methods Podocytes were divided into three groups:Dex group [1 μmol/L Dex +50 mg/L puromycin aminonucleoside (PAN)],PAN group (50 mg/L) and normal control group.Scrape wound assay and Transwell migration assay were used to detect cell motility.Filtering ratio of podocytes was measured by FITC labeled BSA.Real-time PCR and Western blotting were used to examine the expressions of c-actinin-4 and nephrin.Results From the scrape wound assays,the ability of wound repair in podocytes of PAN group was significantly increased (P＜0.01),and the number of migrating cells in this group also rose (P＜0.01).Compared to PAN group,podocytes in Dex group did not enhance the motility after treatment with the same dose PAN (P＜0.01).Real-time PCR and Western blotting showed that Dex could significantly inhibit the up-regulated expression of α-actinin-4 and nephrin induced by PAN.Conclusions Dex can relieve the enhanced motility induced by PAN.Its mechanism may be associated with the modulation of the expressions of α-actinin-4 and nephrin.
