Chymase expression in deep partial-thickness burn wounds after burn injury in hamsters
10.3969/j.issn.1673-8225.2011.46.031
- VernacularTitle:仓鼠深Ⅱ度烧伤创面愈合过程中糜蛋白酶的表达
- Author:
Xianglin DONG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2011;15(46):8681-8684
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Little is known about mast cell chymase in burn injury in hamsters.OBJECTIVE: To investigate whether mast cell chymase is involved in burn injury and the change of chymase expression in burnwound.METHODS: Deep partial-thickness scald in hamsters was induced with contact time of 12 seconds by 20 mL 75 ℃ water in a50 mL syringe. Chymase mRNA level and chymase activity were analyzed before burn and at 1, 3, 7, 14 days after burn in deepsecond-degree scald wound. RESULTS AND CONCLUSION: Quantitative real-time RT-PCR and radioimmunoassay results showed that chymase geneexpression and chymase activity in burn wound were significantly increased after burn and were highest at 3 days after burn.Expression of mast cell chymase would be involved in burn injury and changed at different stages after burn in hamsters.
