Puerarin combined with estradiol for treatment of osteoporosis in ovariectomized rats
10.3969/j.issn.1673-8225.2011.46.035
- VernacularTitle:葛根素联合雌二醇对去卵巢大鼠骨质疏松症的治疗作用
- Author:
Hai LI
;
Jinhua WANG
;
Hailing HUANG
;
Biao LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2011;15(46):8698-8701
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Estradiol produces great adverse effects in treatment of postmenopausal osteoporosis.OBJECTIVE: To investigate the effects of small dose of estradiol combined with estradiol on bone tissue of ovariectomized rats.METHODS: A total of 120 healthy female rats were randomly and evenly divided into five groups: sham-operated (only resectionof a small block of para-ovarian fat pad), ovariectomized (without drugs), puerarin, estradiol, and puerarin + estradiol. One weeklater, rats from the puerarin, estradiol and puerarin + estradiol groups were subcutaneously injected with puerarin (50 mg/kg, oncea day), estradiol (200 μg/kg, twice a week), estradiol puerarin (25 mg/kg, once a day) + (100 μg/kg, twice a week), respectively.RESULTS AND CONCLUSION: In the ovariectomized group, rat bone tissue was sparse, bone calcium and phosphorus levelsas well as bone mineral density were significantly lower compared with sham-operated group (P < 0.01). After estradiol and/orpuerain treatment, rat bone tissue morphology was obviously improved, and bone calcium and phosphorus levels and bonemineral density were significantly increased. The above-mentioned indices were similar after small dose of puerarin combinedwith estradiol treatment and high dose of puerarin combined with estradiol treatment. These findings suggest that small dose ofestradiol combined with estradiol treatment yields satisfactory curative effects in treatment of osteoporosis in ovariectomized rats.
