Olfactory ensheathing cell viability in different cryopreservation systems
10.3969/j.issn.1673-8225.2011.46.024
- VernacularTitle:不同保存方式下嗅鞘细胞的活性
- Author:
Yuliang LIU
;
Kaibin WEI
;
Hong LIU
;
Yuqin HE
;
Feng ZHUO
;
Xingang Lü
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2011;15(46):8653-8656
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: A proper preservation method would be of important significance for experiments and clinical application ofolfactory ensheathing cells (OECs) OBJECTIVE: To explore proper cyropreservative systems for OECs.METHODS: OECs during the logarithmic growth phase were harvested, cryopreserved for 1, 3 and 6 months and then revitalized.RESULTS AND CONCLUSION: MTT assay and tryplan blue staining showed that cells exhibited highest viability after treatmentwith 5% dimethyl sulfoxide (DMSO)-6% hydroxyethyl starch (HES), followed by 10% DMSO, and lastly the 5% DMSO. Use ofrefrigerator or cryogenic control system with different cryopreservation time did not yield obvious effects on viability of OECs.Therefore, 5% DMSO-6%HES is recommended as a cryopreservative agent for OECs.
