A New Indicator Cell Line Established to Monitor Bovine Foamy Virus Infection
- Author:
Hongyan GUO
;
Zhibin LIANG
;
Yue LI
;
Juan TAN
;
Qimin CHEN
;
Wentao QIAO
- Publication Type:Journal Article
- Keywords:
Bovine foamy virus;
Firefly luciferase;
Indicator cell line
- From:
Virologica Sinica
2011;26(5):315-323
- CountryChina
- Language:Chinese
-
Abstract:
In order to improve the accuracy for quantitating the bovine foamy virus(BFV)in vitro,we developed a baby hamster kidney cell(BHK)-21-derived indicator cell line containing a plasmid that encodes the firefly luciferase driven by the BFV long terminal repeat promoter(LTR,from -7 to 1012). The BFV titer could be determined by detecting the luciferase expression since the viral trans-activator BTas protein activates the promoter activity of the LTR. One clone,designated BFVL,was selected from ten neomycin-resistant clones.BFVL showed a specific and inducible dose-and time-dependent luciferase activity in response to BFV infection.Although the changes in luciferase activity of BFVL peaked at 84 h post infection,it was possible to differentiate infected and uninfected cells at 48 h post infection. A linear relationship was established between the multiplicity of infection(MOI)of BFV and the activated ratio of luciferase expression in BFVL. Moreover,the sensitivity of the BFVL-based assay for detecting infectious BFV was 10,000 times higher than the conventional CPE-based assay at 48 h post infection. These findings suggest that the BFVL-based assay is rapid,easy,sensitive,quantitative and specific for detection of BFV infection.
